Dr. Yves Charron

Dr. Yves Charron is an IVF Laboratory Director at the Center for Reproductive Medicine, Kinderwunschärzte Berlin GbR and an ESHRE Certified Senior Clinical Embryologist. Dr. Charron's professional skills include: IVF-Technics: Oocyte pick-up, IVF/ICSI, TESE, oocyte- and PN-scoring, culture of embryos and blastocysts, 2PN cryopreservation and thawing, vitrification/warming, embryo scoring, embryos transfer, Assisted Hatching, polar body, and Trophoectoderm biopsy. Andrologie: Semen anal...ysis, cryopreservation and thawing of gametes (sperm), sperm preparation for IVF/ICSI and insemination. Hormonanalysis: Basic knowledges for the running of the hormone analyser Immulite 2000 XPi (Siemens) Molecular biology: Generation of Drosophila transformant lines by micro-injection, generation of knock out and transgenic mouse lines, RNAi techniques, quantitative RT-PCR, in situ hybridization, Southern and Northern blot, in vitro transcription and translation, cloning. Biochemistry: Recombinant proteins expression and purification, endogenous protein purification by chromatography, protein iodination, enzyme binding assay, zymography, polyclonal antibody purification, Western blot. Cellular biology: In vitro cell culture, Embryonic Stem Cell culture, transient and stable transfections/electroporation, immunohistochemistry, histology.